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Pulivarthi Rao, PhD

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The main goal of Dr. Pulivarthi Rao’s laboratory is the analysis of the genetic basis of deregulation of cellular proliferation and differentiation associated with malignant transformation.

The laboratory staff investigates several tumor types, most notably osteosarcoma and multiple myeloma. A wide range of functional, comparative and cytogenetic techniques is utilized in these studies, which include conventional and molecular cytogenetics- FISH, comparative genomic hybridization (CGH), multicolor spectral karyotyping (SKY), and array CGH. The laboratory emphasizes the identification of oncogenes from the amplified chromosomal regions and genomic insatbility in osteosarcoma.

His laboratory is also focusing on the development of molecular cytogenetic techniques for the analysis of chromosomal aberrations in murine models of human cancers (Comparative Cancer Cytogenetics).

Methods FISH by indirect labeling Direct Labeling for FISH Paraffin FISH Protocol for Q Fish Preparation of human lymphocyte metaphase chromosomes CGH Protocol Multicolor Spectral Karyotyping

Links Mouse Nomenclature Human Gene Nomenclature Guidelines Molecular Cytogenetics Resources SKY/m-FISH and CGH database Cancer genetics NCBI